Journal: Frontiers in Endocrinology
Article Title: Biphasic expression of thyroid hormone receptor TRβ1 in mammalian retina and anterior ocular tissues
doi: 10.3389/fendo.2023.1174600
Figure Lengend Snippet: Cone gene expression in TRβ1-KO mice. (A, B) , Opsin immunostaining (magenta, in outer segments) in retinal cryosections showing superior and inferior views in control (wt) and TRβ1-KO ( Thrb b1/b1 ) adult mice (~2 months old). Fields of view represent 100 μm lengths of ONL (for locations see B) . For comparison, a representative TRβ2-KO displays severe loss of M opsin and extended expression of S opsin in all areas. PNA, peanut agglutinin, pan-cone marker. Rho, rod photopigment, rhodopsin. Dark blue, nuclear (DAPI) stain, shows tissue background. (C) , Counts of opsin- and PNA-positive cones. Mean ± S.D., adult mice; 10 views from the superior and 10 from the inferior retina in the vertical plane around the mid-retinal region obtained from 6 retinas from 3 mice; *p < 0.05, unpaired t-test. (D) , Cone and rod gene mRNA expression analyzed by qPCR. Control mice display counter-gradients of M ( Opn1mw ) and S ( Opn1sw ) opsin mRNA over the superior - inferior plane. In TRβ1-KO mice, Opn1mw and Arr3 are marginally decreased in the inferior retina and Opn1sw modestly elevated in the superior. Most genes show little or no change. Groups, n = 4 mice, with 2 retinas pooled per mouse per region; mean ± S.D., *p < 0.05; **p < 0.001, unpaired t-test on log transformed expression data. (E) , TRβ2 mRNA expression is unchanged in TRβ1-KO mice shown at 2 ages (mean ± S.D., p = 0.58 (P19) and p = 0.16 (P30), unpaired t-test, log transformed expression data); n = 4 retinas representing at least one retina from each of 3 mice. TRβ1-KO lacks TRβ1 mRNA as expected. (F) , TRβ1 mRNA expression is unchanged in TRβ2-KO mice, shown at P30 (mean ± S.D., p = 0.61, unpaired t-test on log transformed expression data); n = 5 retinas representing at least one retina from each of 3 mice. ONL, outer nuclear layer; OS/IS, outer/inner segments.
Article Snippet: Lectin : Rhodamine Peanut Agglutinin (PNA), 10 μg/ml (Vector Lab, RL-1072, RRID : AB_2336642);
Techniques: Expressing, Immunostaining, Control, Comparison, Marker, Staining, Transformation Assay
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